Welcome to our website! Here we briefly introduce the projects we are currently running. We work in multi-disciplinary teams on various topics with exosome biology as underlying focus. Our translational studies include the role of exosomes in cancer biology, metastasis, mesenchymal stem cell biology, oncogenic herpesviruses, microRNAs, innate immunity and auto-immune diseases. We use innovative mouse models, advanced live-imaging techniques, spectral flow cytometry, next generation sequencing and proteomics to unravel the underpinnings of exosomes in health and disease with the aim to translate this knowledge into clinical applications.
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Latest NEWS 15-12-2022 Michiel was awarded a NWO grant with Martine Smit (VU) entitled "Viral GPCR-activated EV release as a novel therapeutic target in brain cancer". 1-12-2022 Rubina officially promoted to PI at the Amsterdam UMC 18-12-2022 Steven and Enzo were selected for oral presentations at NLSEV 2020 in Maastricht, next year ' NLSEV Rotterdam 2023! 22-9-2022 Finally in a great team effort with the labs of Guillaume van Niel and Frederik Verweij we published our exosomes-biogenesis work in JCB using the increasingly popular EV-pHluorin reporter strategy. ER membrane contact sites support endosomal small GTPase conversion for exosome secretion. https://doi.org/10.1083/jcb.202112032 |
Projection of exosome release events (yellow spots) recorded by live TIRF microscopy in a cervical cancer cell (blue) over a time-course of 3 minutes. (Verweij et al JCB 2018). For this Frederik Verweij and Michiel designed a CD63-pHluorin construct that was recently awarded with the Addgene blue flame award as 100 samples have been distributed around the world! We also generated CD9 and CD81 versions that are available at Addgene. https://www.addgene.org/130901/
Steven en Enzo giving talks at NLSEV 2022 in Maastricht
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